Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak ...

Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the ...

It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types of protein ...

Western blot is an invaluable lab technique used to detect proteins in a tissue or blood sample. It helps researchers identify specific protein molecules in a complex mixture of proteins. Since ...

Aldrin V. Gomes, Ph.D. Ning Liu Ph.D. A closer examination of housekeeping and other normalization techniques. In recent years, the scientific community has questioned the reliability of Western ...

Western blots have been a tried-and-true method for separating and quantifying proteins based on molecular weight, but they have not kept up with the continually increasing output demands of modern ...

Post-translational modifications alter protein structure, affecting activity, stability, localization and/or binding partners. Antibodies that specifically recognize post-translationally modified ...

A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same tools ...